Michael Huerta, administrator of the Federal Aviation Administration (FAA), will speak at the runway’s commissioning on August 22.
The FAA funded almost 63% of the $140m project, which was finished on schedule and under original budget, according to the airport.
Port Columbus has several major projects underway to cater for long-term growth.
A three-year $80m programme is overhauling the gateway’s 55-year-old terminal, ensuring it functions for at least 20 more years.
The former south runway, which includes sections of pavement from 1928, will be converted to a taxiway after the new runway opens.